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artificial gene synthesis service  (Thermo Fisher)


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    Structured Review

    Thermo Fisher artificial gene synthesis service
    Artificial Gene Synthesis Service, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/artificial+gene+synthesis+service/gene+synthesis/us12241061-979-41-41
    Average 90 stars, based on 1 article reviews
    artificial gene synthesis service - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Method for treating cancer using artificial adjuvant cell (aAVC)
    Article Snippet: By introducing those genes into FreeStyleTM 293-F cells (Thermo Fisher Scientific, Cat. R79007) using said lentivirus carrying each of the genes, aAVC expressing CD1d and WT1 and loaded α-GalCer onto the cell surface was constructed. (1) Construction of Plasmids for Lentivirus Preparation A gene having a 5′-terminally added XhoI recognition sequence and a 3′-terminally added NotI recognition sequence in the WT1 gene (SEQ ID NO: 1) was inserted into the XhoI-NotI site of pLVSIN-CMV Pur plasmid (Takara Bio Inc., Cat. 6183) to construct pLVSIN-CMV-WT1 plasmid. .. The gene of SEQ ID NO: 1 was prepared by optimization for human codons through the artificial gene synthesis service of Thermo Fisher Scientific Inc. based on the amino acid sequence of Wilms tumor protein isoform D (NCBI Reference Sequence: NP_077744.3). .. By introducing those genes into FreeStyleTM 293-F cells (Thermo Fisher Scientific, Cat. R79007) using said lentivirus carrying each of the genes, aAVC expressing CD1d and WT1 and loaded α-GalCer onto the cell surface was constructed. (1) Construction of Plasmids for Lentivirus Preparation A gene having a 5′-terminally added XhoI recognition sequence and a 3′-terminally added NotI recognition sequence in the WT1 gene (SEQ ID NO: 1) was inserted into the XhoI-NotI site of pLVSIN-CMV Pur plasmid (Takara Bio Inc., Cat. 6183) to construct pLVSIN-CMV-WT1 plasmid.

    Article Title: Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.
    Article Snippet: The nucleotide-binding domain, leucine-richcontaining family, pyrin-domain containing-3 (NLRP3) inflammasome comprises the major components caspase-1, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and NLRP3.. NLRP3 plays important roles in maintaining immune homeostasis mediated by intestinal microorganisms and in the immunostimulatory properties of vaccine adjuvants used to induce an immune response.. In the present study, we first cloned a complementary DNA (cDNA) encoding porcine ASC because its genomic sequence was not completely determined.

    Article Title: Method for treating cancer using artificial adjuvant cell (aAVC)
    Article Snippet: The amplified CD1d gene was inserted into the cleaved plasmid described above using In-Fusion® HD Cloning Kit (Takara Bio Inc., Cat. 639648) to construct pLVSIN-CMV-CD1dΔpur plasmid. .. The gene of SEQ ID NO: 3 was prepared by optimization for human codons through the artificial gene synthesis service of Thermo Fisher Scientific Inc. based on the amino acid sequence (SEQ ID NO: 4) of antigen-presenting glycoprotein CD1d isoform 1 precursor (NCBI Reference Sequence: NP_001757.1). .. The amplified CD1d gene was inserted into the cleaved plasmid described above using In-Fusion® HD Cloning Kit (Takara Bio Inc., Cat. 639648) to construct pLVSIN-CMV-CD1dΔpur plasmid.

    Wilms Tumor Assay:

    Article Title: Method for treating cancer using artificial adjuvant cell (aAVC)
    Article Snippet: By introducing those genes into FreeStyleTM 293-F cells (Thermo Fisher Scientific, Cat. R79007) using said lentivirus carrying each of the genes, aAVC expressing CD1d and WT1 and loaded α-GalCer onto the cell surface was constructed. (1) Construction of Plasmids for Lentivirus Preparation A gene having a 5′-terminally added XhoI recognition sequence and a 3′-terminally added NotI recognition sequence in the WT1 gene (SEQ ID NO: 1) was inserted into the XhoI-NotI site of pLVSIN-CMV Pur plasmid (Takara Bio Inc., Cat. 6183) to construct pLVSIN-CMV-WT1 plasmid. .. The gene of SEQ ID NO: 1 was prepared by optimization for human codons through the artificial gene synthesis service of Thermo Fisher Scientific Inc. based on the amino acid sequence of Wilms tumor protein isoform D (NCBI Reference Sequence: NP_077744.3). .. By introducing those genes into FreeStyleTM 293-F cells (Thermo Fisher Scientific, Cat. R79007) using said lentivirus carrying each of the genes, aAVC expressing CD1d and WT1 and loaded α-GalCer onto the cell surface was constructed. (1) Construction of Plasmids for Lentivirus Preparation A gene having a 5′-terminally added XhoI recognition sequence and a 3′-terminally added NotI recognition sequence in the WT1 gene (SEQ ID NO: 1) was inserted into the XhoI-NotI site of pLVSIN-CMV Pur plasmid (Takara Bio Inc., Cat. 6183) to construct pLVSIN-CMV-WT1 plasmid.

    Positive Control:

    Article Title: Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.
    Article Snippet: The nucleotide-binding domain, leucine-richcontaining family, pyrin-domain containing-3 (NLRP3) inflammasome comprises the major components caspase-1, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and NLRP3.. NLRP3 plays important roles in maintaining immune homeostasis mediated by intestinal microorganisms and in the immunostimulatory properties of vaccine adjuvants used to induce an immune response.. In the present study, we first cloned a complementary DNA (cDNA) encoding porcine ASC because its genomic sequence was not completely determined.

    Synthesized:

    Article Title: Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.
    Article Snippet: The nucleotide-binding domain, leucine-richcontaining family, pyrin-domain containing-3 (NLRP3) inflammasome comprises the major components caspase-1, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and NLRP3.. NLRP3 plays important roles in maintaining immune homeostasis mediated by intestinal microorganisms and in the immunostimulatory properties of vaccine adjuvants used to induce an immune response.. In the present study, we first cloned a complementary DNA (cDNA) encoding porcine ASC because its genomic sequence was not completely determined.

    Clone Assay:

    Article Title: Identification of the Q969R gain-of-function polymorphism in the gene encoding porcine NLRP3 and its distribution in pigs of Asian and European origin.
    Article Snippet: The nucleotide-binding domain, leucine-richcontaining family, pyrin-domain containing-3 (NLRP3) inflammasome comprises the major components caspase-1, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and NLRP3.. NLRP3 plays important roles in maintaining immune homeostasis mediated by intestinal microorganisms and in the immunostimulatory properties of vaccine adjuvants used to induce an immune response.. In the present study, we first cloned a complementary DNA (cDNA) encoding porcine ASC because its genomic sequence was not completely determined.

    other:

    Article Title: Method for treating cancer using artificial adjuvant cell (aAVC)
    Article Snippet: Mouse-type aAVC (also referred to as aAVC(3T3)-WT1) was prepared by introducing mRNAs of the WT1 gene and the CD1d gene into mouse NIH/3T3 cells (ATCC, Cat. CRL-1658), and adding α-GalCer (synthesized by Juzen Chemical Corp. under manufacturing agreement) thereto.



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